Supercritical Fluid Extraction with CO2

Extraction of natural biologically active substances using innovative and nature-friendly technology

síbiotech specialises in extracting natural biologically active compounds using an innovative and environmentally friendly technology – supercritical fluid extraction with carbon dioxide (CO2). Biologically active compounds are obtained from various natural raw materials, including berries, fruits, microalgae, and medicinal plants, as well as by-products of their processing, such as sea buckthorn seeds, hemp seeds, flaxseeds, cranberries, chokeberries, and more.

The obtained extracts do not contain CO2 residues. CO2 is separated from the extract by transformation back into a gaseous state. These extracts are high quality, microbiologically stable, and have a long shelf life. Compared to conventional technologies, the technology is environmentally friendly and avoids using organic solvents.

From raw materials, both fat-soluble and polar compounds are extracted through supercritical fluid extraction, employing carbon dioxide and a for polar fractions co-solvent, ethanol. Carbon dioxide (CO2) and ethanol are recognised as safe and harmless solvents (GRAS). In the supercritical fluid extraction process, carbon dioxide is subjected to pressure (74 bar) and temperature (31°C) above its critical limits. Under these conditions, CO2 changes its physicochemical properties, entering a supercritical state and acquiring efficient solvent characteristics.

Circulating through the raw material at a specific quantity and speed, CO2 extracts fat-soluble compounds such as oils, fats, and waxes. During the extraction, CO2 saturated with the compounds in the raw material is directed to separators, where the pressure is reduced, causing CO2 to return to a gaseous state. This process yields specific fractions of biologically active compounds. The liberated CO2 from the separators is recycled and used for subsequent extraction cycles.

Advantages of the Technology
Advantages of the Technology
It is considered a "solvent-free" technology
Safe, gentle, and sustainable
High concentrations of fat-soluble bioactive compounds
Extracted bioactive compounds are in their natural chemical form, not chemically modified (triglycerides, phospholipids)
No degradation of bioactive compounds during extraction (low temperature)
No contact with oxygen during the process, preventing oxidation of fatty acids (polyunsaturated fatty acids oxidise quickly)
Microbiologically stable extracts (high pressure during extraction)
Extracts do not contain proteins, fibers, inorganic salts, or allergens
Extracts are free from harmful impurities, such as heavy metals, ash, etc.
Using the secondary solvent ethanol (up to 5%), it is possible to obtain polar bioactive compounds.
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Supercritical Fluid Extraction with CO2 is an Effective and Environmentally Friendly Method

Biologically active compounds are obtained from natural raw materials – berries, fruits, microalgae, and medicinal plants, as well as by-products of their processing (press cakes, seeds, etc.), such as sea buckthorn seeds, hemp seeds, flaxseeds, cranberries, chokeberries, and more.
Both fat-soluble biologically active compounds, such as fatty acids and lipid extracts, and polar groups of biologically active compounds, such as polyphenols, are obtained.
CO2 and ethanol are recognised as safe and harmless solvents. Obtained extracts do not contain CO2 residues; they are of high quality, microbiologically stable, and have a long shelf life.

Usage of Extracts Obtained with Innovative Technologies

In herbal pharmaceutical preparations
Cosmeceuticals
Nutraceuticals
Functional food ingredients
Usage of Extracts Obtained with Innovative Technologies

HIPPOCOR® – A Product Created Using Innovative Technology

HIPPOCOR® is a health-promoting product created by our team of scientists and cardiologists. It is produced from sea buckthorn seeds using supercritical fluid extraction with CO2.

Thanks to innovative technology, a high-quality sea buckthorn seed lipid extract is obtained, which contains Omega-3, Omega-6, and Omega-9 fatty acids and beta-sitosterol.

This innovative method is gentle; hence, fatty acids are not chemically modified or processed with organic solvents, as is common in other products. The obtained lipid extracts are microbiologically stable and have a long shelf life; they are free from heavy metals, inorganic salts, proteins, fibers, and allergens. A significant advantage of the technology is that there is no contact with oxygen during the extraction, reducing the risk of fatty acid oxidation and preserving the biological activity and integrity of the product.

Collaboration

síbiotech develops and implements new technologies and solutions for extracting and refining bioactive compounds. If you need natural bioactive compounds, let us know! Our team will be happy to collaborate with you!

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